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Subsequently, the tube sample was treated with 5 mL of pre-warmed Trypsin (Gibco, 15050065), 4 mL of pre-warmed HBSS (Servicebio, G4202), and 100 L of DNase I (Sigma, DN25, 5 mg/mL), followed by shaking at 120 rpm for 8 min at 34 C